Elements of Harmony
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Depiction in the series
Friendship is Magic, part 1 and part 2

Added by Reversalmushroom
Added by Reversalmushroom
Added by Xx Mr Brony xXThe segment showing Elements of Harmony in the prologue has gems colored as follows, clockwise from the top: red, orange, green, blue, pink, and the center gem colored purple. When Twilight and her friends use the elements, there is no green gem, and instead there is a magenta-colored gem that is bestowed upon Twilight.
The pony reading this legend, Twilight Sparkle, sets to learn more about Night Mare Moon and the Elements of Harmony, and discovers that Night Mare Moon is set to return "on the longest day of the thousandth year", which is in two days' time. She sends a letter to Princess Celestia warning her of Night Mare Moon's return, but the Princess tells her to "stop reading those dusty old books" and sends her to Ponyville to see to the preparations of the Summer Sun Celebration and "make some friends".
After overseeing the preparations and inadvertently making friends, the moment of Night Mare Moon's return arrives, and Princess Celestia goes missing. Twilight sets out to find the Elements of Harmony to defeat Night Mare Moon, and her new friends accompany her. She discovers that they are located in the ruins of the "ancient castle of the royal pony sisters" which is located in the Everfree Forest. They each demonstrate a noble quality throughout their journey, which is later linked to an Element of Harmony. They come upon five of the Elements of Harmony (briefly shown to be six) which are shown as stone orbs. They are later transformed through Twilight's magic into gem-studded jewellery, all of them matching the cutie mark of the wearer.Applejack – Honesty

Added by PonyNewsFluttershy – Kindness

Added by PonyNewsPinkie Pie – Laughter

Added by PonyNewsRarity – Generosity

Added by PonyNewsRainbow Dash – Loyalty

Added by PonyNewsTwilight Sparkle – Magic

Added by PonyNewsThe Return of Harmony Part 1 and Part 2
Princess Celestia tells Twilight and her friends in The Return of Harmony Part 1 that the Elements were wielded by herself and Princess Luna and were used to defeat Discord by turning him in to stone. She continues, saying that she and her sister are no longer connected to the Elements. She stores the Elements in what she believes to be a chamber only she can open with her magic spell, but upon opening it she discovers the Elements are missing, and that Discord has hidden them somewhere. Discord reveals the whereabouts of the Elements in a riddle, and Twilight and her friends venture on a quest to retrieve them. Discord makes them behave in a manner contrary to the Element they represent, and once they find the Elements they are not able to use their magic.
Added by ReversalmushroomAfter the failed attempt, Twilight is about to give up, but then discovers that Celestia has been sending back all her friendship reports. She reads the friendship reports and reverts to her former self, then sets out to use a memory spell on each of her friends, which restores them to their former selves too. They confront Discord again, this time successfully turning him to stone with the magic of the Elements and restoring Ponyville and Equestria to their former state.
A Canterlot Wedding - Part 1 and Part 2
The Elements of Harmony are mentioned again in the season two finale, A Canterlot Wedding. After being defeated by Queen Chrysalis, Princess Celestia instructs the six ponies to retrieve the Elements from Canterlot Tower to stop Chrysalis and her changeling army. Despite their efforts, they are unable to accomplish the task as the changelings have taken hold of all of Canterlot, including the tower.
Other depictions
Twilight Sparkle is sold with her element of magic tiara in the Royal Castle Friends toy set.
The 2012 trading cards mention a seventh Element of Harmony, Love, which is attributed to Princess Cadance and Shining Armor.