A background pony on page 6 resembles actor Donald Sutherland, making a gesture identical to one made by Sutherland's character in the 1978 film Invasion of the Body Snatchers.
Book titles seen in Twilight's library on page 7 include "To Serve Ponies" (a reference to the 1950 science fiction short story "To Serve Man" and the 1962 Twilight Zone episode of the same name), "How I Did It" (a reference to 1974's Young Frankenstein), and "Incident at Santa Mira" (another reference to Body Snatchers).
Rainbow Dash's cry of "It's clobbering time!" is the catchphrase of Marvel Comics' The Thing.
The scene where Pinkie Pie teaches the characters how to act like changelings is a reference to a scene in Shaun of the Dead.
Rainbow Dash telling Fluttershy to be "nice until it's time not to be nice" is a reference to Road House.
The Secretariat Comet is named for renowned real-life racehorse Secretariat.
A gray background pony on page 17 resembles an Observer from the science fiction TV series Fringe.
When the group stands in front of the entrance to the cave, the names under the two gargoyles are "Ziggy" and "Stardust", a reference to the stage name used by British rock musician David Bowie.
Queen Chrysalis's chambers are an allusion to the Wicked Witch of the West's from the 1939 film The Wizard of Oz.
Also, the panel that Queen Chrysalis send out the changelings mirrors the scene where the Wicked Witch of the West sends out the flying monkeys.
This issue contains references to the song "Diamond Dogs" by David Bowie, such as a sign on page 10 reading "sashay on the boardwalk, scurry to the ditch". Another sign references Bowie's song "Cat People (Putting Out the Fire)": "Red like jungle burning bright". The mine's exit reads "Never look back, walk tall, act fine", lyrics from "Golden Years".
The "thwip" sound effect used by the spiders' web mirrors that of Marvel Comics' Spider-Man.
Landmarks on the map at the end of the issue include the Swamps of New Horseleans, an allusion to real-life city New Orleans, and Rocinante Cliffs, a reference to Don Quixote's horse.
On page 18, Spike cradles a blanket and sucks his thumb in a manner identical to Linus van Pelt from Charles M. Schulz' Peanuts comic strip.
Twilight's line "That's one small step for ponies, one giant leap for ponykind!" is a reference to the famous words Neil Armstrong spoke when he set foot on the moon in 1969.
Pinkie's line "In space, no one can hear you squee!" is a reference to the tagline for 1979's Alien.
Two Dr. Hooves appear standing next to each other in the last panel on page 3, one with a messy mane commonly associated with "Ten", the other with a swoopy mane, a fez, and a bow-tie characteristic of "Eleven".
It is notable that the Doctor Who 50th anniversary special, The Day of the Doctor, similarly features both Ten and Eleven working together and debuts on the same date as Season 4 of Friendship is Magic.
Similar to issue 7, two Dr. Hooves appear standing next to each other in the same panel on page 10; one is "Ten," and the one next to him with a curly mane and tail and a very long scarf is "Four."
On page 15 he is wearing a tie around his head, similar to his appearance in a popular scene from the Doctor Who episode, The Girl in the Fireplace.
Granny Smith's pose on page 11 resembles the "Crane Stance" demonstrated by Ralph Macchio's character in The Karate Kid. Furthermore, Big McIntosh's exclamation of "By the mane of Miyagi!" is a reference to Pat Morita's character Keisuke Miyagi from the same film.
A green pony with a dark blue scarf who looks like Sherlock Holmes (as played by Benedict Cumberbatch in Sherlock) appears on page 16.
Poindexter is seen also playing a cowbell,a reference to Saturday Night Live Sketch "More Cowbell."
Poindexter's synthesizer has the label: "The Mystic Knights of the Electric Stable" referencing Oingo Boingo 's original name: "The Mystic Knights of Oingo Boingo" as well as having a sticker saying "Who Care What Frankie Say?", referencing the band Frankie Goes To Hollywood 's famous T-Shirt slogan: "Frankie Say Relax Don't Do It!
A number of the gadgets and products that Shining Armor and his friends use to try and sabotage Buck Withers bear the Acme logo.
In the top right corner of page 17, there is a banner that says "M*A*S*H the Manticores". The word MASH is written in a fashion that references the television show of the same name.
The subtitle for neigh anything Part 2, "presentable in periwinkle", is a play on the title of the 1986 film Pretty in Pink.
On page 8, Princess Cadance is holding an album called "Magenta Rain" by a band called Prance and the Revolution, a play on Purple Rain by Prince and The Revolution.
An album on page 9 bears the name Bruce Springsteed, a clear reference to singer-songwriter Bruce Springsteen.
Also on page 9 is an album by a band called Stallions at Work, a play on Men at Work. The album cover bears a resemblance to Men at Work's "Business as Usual".
On page 10 are posters of The New Foal Titans, an allusion to DC Comics' Teen Titans, and the Wylld Stallyns, a reference to the fictional band in the Bill & Ted films.
On page 11, 8-bit mentions a game called "Hocuspocus: The Get-Together", a play on Magic: The Gathering.
On the same page, Poindexter says "Thanks for the sour persimmons, buster", a near verbatim variation of a line spoken by Daffy Duck in the Looney Tunes animated short Duck Amuck.
On page 18, Shining Armor mentions Gaffer's replica bat'leth sword. A bat'leth is a traditional Klingon bladed weapon in the Star Trek franchise.
Also on the page, Gaffer and 8-Bit are shown planning a prank on Buck Withers. The prank involved a bucket but they decide not to do it because it was "Too messy". This is a reference to the moment in the Stephen King novel Carrie when a bucket of blood is dropped on the prom queen.
On page 21, Gaffer's line "Why haven't I leaped, Al?" is a reference to the science-fiction series Quantum Leap. The page also features a pony bearing a resemblance to Quantum Leap character Al Calavicci.
In the short post-comic section PTV by Katie Cook, there are an additional ten musician and song references:
In the Robin Hood-like book, Rarity takes on a role similar to Rapunzel. On page 21, Rainbow Dash calls her "Ms. Lost Princess", a reference to Tangled, the Disney adaptation of the Rapunzel fairy tale.
The fantasy story Pinkie creates shares several parallels with The Lord of the Rings, such as the story's title "One Worm To Fool Them All", Pinkie being dressed similarly to Gandalf, Rainbow Dash being dressed similarly to Frodo Baggins, and Mt. Worm.
Rainbow Dash's comment of 'we're not using the zed word' is a reference to British 2004 horror comedy Shaun of the Dead
The sci-fi story Rainbow Dash creates shares parallels with Star Trek, including Rarity's similar appearance to Nyota Uhura and Twilight's similar appearance to a Vulcan.
On page 1, the Observer pony says "Twilight Sparkle... there is more than one of everything." This alludes to the first season finale of Fringe which had a similar plot (a portal to a parallel universe).
On page 8, the helmet display case marked "Helm of Neigh-bu" alludes to an identical helmet worn by DC Comics' Doctor Fate, whose helmet was obtained by the ancient Egyptian wizard Nabu.
On page 10, there is a dismantled golden handgun in a display case. This item is a reference to Francisco Scaramanga's signature weapon from the James Bond film, The Man with the Golden Gun.
On page 19, Rainbow Dash is reading a book that appears to be Bram Stoker's Dracula. The quote on the page she's on reads "Welcome to my house. Come freely. Go safely; and leave something of the happiness you bring!"
Throughout the issue, Princess Luna and Spike are seen playing various Hasbro-owned board games or variants thereof:
Another poster reads "Mane", and resembles the poster for the controversial 60's counterculture musical Hair.
On page 15, the two rightmost ponies in panel 3 are based on the characters Venus Flytrap and Dr. Johnny Fever from WKRP in Cincinnati.
On page 20, pony versions of an Observer from Fringe and Tara Strong can be seen.
One of the pony hippies on page 7 resembles cult leader Charles Manson.
Prior to Pinkie Pie bursting into the library on page 1, there are vibrations in Twilight's glasses of water, a reference to a scene in 1993's Jurassic Park.
Pinkie Pie mentions winning her tickets to see Ponyacci from a contest held by Colta-Cola, a reference to the soft drink Coca-Cola.
The issue features a clown pony named Ponyacci, a reference to the Italian opera Pagliacci.
The scene where Pinkie meets Ponyacci mirrors a joke told by Rorschach in Watchmen. In it, a depressed man is advised by a doctor to see the clown Pagliacci to cheer him up, at which the man bursts into tears and reveals that he is Pagliacci.
One of Ponyacci's students near the end of the issue is named Trollo Lollo, a reference to Eduard Khil's rendition of I Am Glad, 'Cause I'm Finally Returning Back Home (or, as it's more popularly known, the "Trololo song").
The name of the Sass Squash is play on that of the fabled sasquatch, or Bigfoot.
Apple Bloom's line, "Magnets! How do they work?" is a reference to the Insane Clown Posse song Miracles.
One of Applejack's lines, "Waitin' is the hardest part!", is a reference to the Tom Petty and the Heartbreakers song The Waiting.
Among the toys Sweetie Belle suggests as a possible transformation for Imp are Octi from the Powerpuff Girls, a Ponyacci doll from the 5th Micro-Series and the Ursa Minor doll from one of the covers of Pinkie Pie's Micro Series
The outfit Apple Bloom models on page 14 features parts of Tom Servo and Crow T. Robot's heads, as well as the dog-bone satellite, from Mystery Science Theater 3000. Scootaloo and Sweetie Belle's lines are also from MST3k.
The two-page flea market panel has a number of cameos from the show including: Daring Do, Lightning Dust, Ms Peachbottom and Aunt Orange. As well as cameos of other characters including: (Ponified) Where is Waldo, Ponyta, Keldeo and Starlight (the Horse from Rainbow Brite)
A number of unicorns featured in the issue bear resemblances to characters of the Harry Potter series:
Furthermore, the red-capped colt's cutie mark appears to be a silhouette of Baby Huey, the blue-capped colt's cutie mark is a book (alluding to the Dewey Decimal system), and the green-capped colt's cutie mark is a trumpet, the signature instrument of Louie Armstrong.
On page 9, panel 3, the red-maned pony with a baseball bat cutie mark resembles Gosalyn Mallard from Darkwing Duck. The stallion accompanying her is wearing a pink shirt and green sweater -- the casual attire of Drake Mallard, her father.
During Princess Celestia's flashback (on page 10), there is a white-haired blue stallion with an hourglass cutie mark whose design resembles that of the first Doctor from the series Doctor Who.
A number of ponies featured in the issue also bear resemblances to characters of the Sailor Moon manga:
The bushy-haired, mustachioed pony calling the meeting to order resembles the main character from TV show Welcome Back, Kotter.
On page 1, Twilight Sparkle calls Owlowiscious' pose "Karate Colt", a reference to The Karate Kid.
Page 4 shows two royal guards passing each other named Sam and Ralph. This is a reference to Warner Bros.' Ralph Wolf and Sam Sheepdog series of cartoon shorts.
On page 17, the pink Crystal Pony has the Star Trek Starfleet operations division insignia as a cutie mark.
Page 18 features the Magnum, P.I. pony from Friendship is Magic Issue #1.
Below the Magnum P.I. pony and to the left of DJ Pon-3, there is a pony with glasses, a red and white striped sweater and hat, and the letter "W" as a cutie mark. This pony and the entire panel is a reference to the Where's Waldo? children's books and activities.
Directly behind Magnum is a frazzled pony with a taxicab-styled cart the resembles "Reverend" Jim Ignatowski from the TV series Taxi.
On page 19, the side of a trailer reads "Faust's Traveling Circus".
Another trailer with a shark tank reads "Bruce the Shark" while showing an image of a Great White Shark. Both the name and type of shark shown reference a character from Finding Nemo.
At the end of page 21, Princess Luna, Apple Bloom, and Luna's pet opossum Tiberius are talking behind a brick wall. This panel closely mimics an activity commonly seen in Charles M. Schulz's Peanuts comic strips.
In Katie Cook's mini-comic that follows Issue #10 titled "Luna Gets a Pet", Fluttershy says "My Spidey Sense is tingling...". This is a quote often said in the Spider-Man comics.
In this mini-comic, Princess Luna almost chooses an owlbear as her pet but changes her mind for fear of "copyright issues". The owlbear is an original Dungeons & Dragons creature.
Princess Luna names her new pet opossum Tiberius, a reference to James Tiberius Kirk of Star Trek.
Page 19 features the Crusaders in a Power Rangers parody, complete with the Crusaders piloting a Megazord and fighting a monster that is portrayed by Gummy.
Page 20 references Lord of the Rings, with Starswirl as Gandalf.
On page 12, Spike says "You could have turned the bars into steam, or the walls into snowflakes or teleported both of us back to Canterlot." This is almost word for word from the scene in Watchmen when The Comedian is attacked by the Vietnamese woman with the bottle.
In the final panel of the comic, the flyer by Sunset Shimmer's foot tells students to bring submissions to 'Megan, Danny or Molly', the main human characters of the classic My Little Pony television series.
On the page where Applejack is trying on various outfits, one of the outfits is the Yellow & Black striped jumpsuit made famous by Game of Death and Kill Bill.