My Little Pony Friendship is Magic: Discover the Difference is a flash game available on the Hubworld, Hasbro, and Treehouse TV websites, as well as on Ziosk restaurant tabletop devices. Players are shown two similar images with five differences, and must use the mouse to click on the differences. Clues are available, but using a clue earns the player a silver star instead of a sapphire star, called "shimmering sparkles" in the game's how-to guide. Earning five stars earns the player a golden medal, or "pony ribbon", for the level. As each difference is clicked, it is changed to match the image on the left.
A limited amount of randomness is built into the game, allowing for variation during replay. Each level (after the first) has eight possible differences, but only five of them are presented during any given session.
The game is sorted into five groups of four images. The following chart numbers them from left to right.
|Image||Related to episode||Brief description|
|1||Friendship is Magic, part 1||Pinkie Pie first meets Twilight Sparkle.|
|2||Friendship is Magic, part 2||The ponies glow as they discover the Elements of Harmony.|
|3||The Ticket Master||Spike is holding up six Grand Galloping Gala tickets for the ponies.|
|4||Applebuck Season||The ponies sit around a table having apple juice.|
|5||Griffon the Brush Off||Rainbow Dash and Pinkie are holding hooves.|
|6||Boast Busters||Twilight's horn glows bright as Trixie looks in fright.|
|7||Dragonshy||Fluttershy disciplines the red dragon.|
|8||Look Before You Sleep||Applejack, Rarity, and Twilight sit on the library floor curling their manes.|
|9||Bridle Gossip||Zecora stands in her cloak in front of poison joke.|
|10||Swarm of the Century||Pinkie leads the parasprites into the Everfree Forest.|
|11||Call of the Cutie||Twilight overlooks the Cutie Mark Crusader's first meeting.|
|12||Fall Weather Friends||Applejack and Dash run through autumn leaves.|
|13||Feeling Pinkie Keen||Twilight and Pinkie are wearing umbrella headgear.|
|14||Sonic Rainboom||Dash is wearing a tiara and being hoisted by her friends.|
|15||The Show Stoppers||The Cutie Mark Crusaders perform their song.|
|16||A Bird in the Hoof||Philomena perches on Celestia's front leg.|
|17||The Cutie Mark Chronicles||The six leading ponies hug.|
|18||Owl's Well That Ends Well||Owlowiscious, Twilight, and Spike stand next to a scroll.|
|19||Party of One||Pinkie sits with her friends around her, wearing party hats.|
|20||The Best Night Ever||Rainbow Dash, Princess Celestia, Twilight Sparkle, Spike, Applejack, Rarity, and Fluttershy stand around a modest table with a single plate of half-eaten donuts.|
Aside from level one–which appears automatically and requires the indicated difference to be clicked first–the levels may be played in any order and do not need to be completed before changing to another.